We explored the effectiveness of oregano, a member of the Lamiaceae family, specifically regarding its ability to combat Salmonella, a major foodborne pathogen. The study focused on several plants, including oregano, and analyzed their phenolic compounds, which have been found to provide both antioxidant and antimicrobial benefits.
Our findings showed that oregano extracts had a remarkable antimicrobial effect, with the ability to inhibit the growth of Salmonella with a minimum inhibitory concentration (MIC) of less than 1. This indicates that oregano is not only safe but also potent in battling bacteria associated with food memories.
Overall, we observed that oregano and other selected plants presented a promising source of natural bioactive ingredients. This could have significant implications for the food and cosmetic industries, offering safe alternatives to synthetic preservatives.
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We investigated the potential of oregano essential oil (EO) to combat Salmonella, specifically focusing on the strain known as serovar Typhimurium. The study assessed how effective the EO is in not only preventing the growth of the bacteria but also disrupting established biofilms—those resilient communities that bacteria form to protect themselves.
Our findings showed that the EO has remarkable antimicrobial properties, with minimum inhibitory concentrations (MICs) between 0.03% and 0.06%. Even more impressive was its ability to eradicate biofilm cells completely at a concentration of 6.25% after just 15 minutes of exposure. Analysis of the EO revealed that its main components include thymol, -cymene, and carvacrol—all known for their antibacterial qualities.
Notably, when tested on planktonic cells, the EO led to a significant reduction in the expression of important virulence genes, indicating its potential to weaken bacteria on multiple fronts. Overall, our results highlight oregano EO as a promising natural alternative for food safety and health applications aimed at tackling foodborne pathogens like Salmonella.
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Oregano Oil’s Antimicrobial PotentialFabrication, characterization, and application of pea protein-based edible film enhanced by oregano essential oil (OEO) micro- or nano-emulsion.
Clear impact on salmonella growth
We explored the potential of using oregano essential oil (OEO) to improve food safety, specifically its effectiveness against salmonella strains. In our study, we incorporated varying amounts of OEO into edible films made from pea protein isolate.
By testing these films, we were able to observe the impact of OEO on the growth of salmonella, particularly three strains: Typhimurium, Infantis, and Enteritidis. Notably, when we added a concentration of 2.0% OEO, we found that it significantly inhibited the growth of these harmful bacteria. Additionally, applying these films to chicken breast resulted in a meaningful reduction in microbial counts.
Though the strength of the films and their barrier properties fluctuated with higher concentrations of OEO, the antimicrobial properties remained impressive. It's fascinating that reducing the OEO particle size didn’t significantly change the film's effectiveness, suggesting that both micro- and nano-emulsions can be effective in fighting bacteria.
Overall, our findings suggest that pea protein-based films integrated with oregano oil could serve as an innovative and effective solution for enhancing food safety, especially for poultry products.
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Oregano oil effectively combats salmonellaOregano Essential Oil versus Conventional Disinfectants against Typhimurium and O157:H7 Biofilms and Damage to Stainless-Steel Surfaces.
Highly relevant salmonella study
We explored the effectiveness of oregano essential oil in breaking down biofilms of two types of salmonella, specifically Typhimurium and O157:H7, that were adhered to stainless steel surfaces. By testing various concentrations of oregano oil, we found that it worked well at concentrations between 0.15 and 0.52 mg/mL, effectively disrupting bacterial biofilms.
In addition to evaluating the efficacy against salmonella, we assessed the impact of different disinfectants on the integrity of the stainless steel surfaces. Oregano oil stood out as it caused the least amount of damage, exhibiting only about 0.001% weight loss after treatment. In contrast, sodium hypochlorite, one of the more conventional disinfectants, inflicted notable harm, resulting in a weight loss of 0.00817% at its effective concentration.
Overall, our findings suggest that oregano essential oil not only effectively eliminates harmful bacteria from surfaces but also does so with minimal risk to those surfaces compared to other disinfectants. This positions oregano as an attractive option for those looking for effective yet gentle cleaning methods against persistent bacterial biofilms.
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Oregano oil inhibits Salmonella growthActivity of plant essential oils against clinically and environmentally isolated Salmonella enterica serotypes: in vitro assays and molecular docking.
High relevance for Salmonella treatment
Our research focused on understanding how oregano essential oil (EO) can combat various strains of Salmonella, including those from clinical and environmental sources. By testing the antimicrobial properties of oregano, thyme, and grapefruit EOs against specific Salmonella serotypes like S. Saintpaul, S. Infantis, and S. Oranienburg, we aimed to uncover effective natural alternatives for food preservation.
The standout result was oregano EO, which not only showcased strong antimicrobial activity but also had a minimum inhibitory concentration (MIC) of just 0.1 μL/mL for all tested Salmonella serotypes. This means it can effectively halt the growth of these harmful bacteria at very low concentrations. Thyme and grapefruit EOs also displayed good activity, particularly against certain clinical serotypes, but oregano was definitely the star of the show.
Additionally, we conducted molecular docking studies to investigate how compounds like thymol from oregano interact with Salmonella enzymes. This analysis revealed promising binding energies, suggesting the potential mechanisms through which oregano can exert its antimicrobial effects.
Overall, our findings indicate that oregano essential oil could serve as an effective natural preservative, helping to tackle Salmonella contamination in food products.
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