Overview
SCIENTIFIC SCORE
Likely Ineffective
Based on 1 Research
4
USERS' SCORE
Good
Based on 7 Reviews
8.3
Supplement Facts
Serving Size: 1 Nugget
Amount Per Serving
%DV
Vitamin B12 (as methylcobalamin)
5,000 mcg
208,333%
Top Medical Research Studies
4
Vitamin B12's unclear impact on glioblastoma
We explored the effects of vitamin B12 (cobalamin) supplementation on a human glioblastoma cell line known as U-87 MG. Our main goal was to understand how cobalamin, particularly in combination with pyridoxine (vitamin B6), influences cell viability and the process of cell death, known as apoptosis.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
Read More
Most Useful Reviews
9
Clarity and energy
Excellent. The best brand I have tried. This is the most effective brand thus far. I have tried numerous brands that simply do not work. This and Jarrow work exceptionally well, but this one truly stands out. It provides clarity and instant energy for my mind. Remarkable for overcoming physical and mental fatigue as well as brain fog. Although I am not keen on the inclusion of mannitol, I can overlook that.
Read More
7.5
Significant rest effects
12 people found this helpful
This product is remarkably different from those I've previously tried. The small pink tablet made me feel significantly sleepy, which my body needed for recovery. After several days of rest, I felt remarkably refreshed!
Read More
9
Hair and nail improvement
10 people found this helpful
This product effectively raised my B12 levels from 270 to 960. I suffered from hair loss, but after a month of a high dosage B12 supplement, my levels improved significantly. I feel a surge of strength and clarity in my brain.
Read More
Medical Researches
SCIENTIFIC SCORE
Likely Ineffective
Based on 1 Research
4
- All Researches
4
Vitamin B12's unclear impact on glioblastoma
We explored the effects of vitamin B12 (cobalamin) supplementation on a human glioblastoma cell line known as U-87 MG. Our main goal was to understand how cobalamin, particularly in combination with pyridoxine (vitamin B6), influences cell viability and the process of cell death, known as apoptosis.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
Read More
User Reviews
USERS' SCORE
Good
Based on 7 Reviews
8.3
- All Reviews
- Positive Reviews
- Negative Reviews
9
Clarity and energy
Excellent. The best brand I have tried. This is the most effective brand thus far. I have tried numerous brands that simply do not work. This and Jarrow work exceptionally well, but this one truly stands out. It provides clarity and instant energy for my mind. Remarkable for overcoming physical and mental fatigue as well as brain fog. Although I am not keen on the inclusion of mannitol, I can overlook that.
Read More
7.5
Significant rest effects
12 people found this helpful
This product is remarkably different from those I've previously tried. The small pink tablet made me feel significantly sleepy, which my body needed for recovery. After several days of rest, I felt remarkably refreshed!
Read More
9
Hair and nail improvement
10 people found this helpful
This product effectively raised my B12 levels from 270 to 960. I suffered from hair loss, but after a month of a high dosage B12 supplement, my levels improved significantly. I feel a surge of strength and clarity in my brain.
Read More
9
Calm through craziness
1 people found this helpful
This B12 gives me great energy and clears brain fog. I feel calmer during hectic days at work, making it the best B12 for me.
Read More
7.5
Repairing brain nerves
1 people found this helpful
I feel positive effects from this high-dose B12 supplement, particularly in helping repair brain nerves and improving my sleep quality. I'm keen to see more pronounced effects.
Read More
Frequently Asked Questions
9
Clarity and energy
Excellent. The best brand I have tried. This is the most effective brand thus far. I have tried numerous brands that simply do not work. This and Jarrow work exceptionally well, but this one truly stands out. It provides clarity and instant energy for my mind. Remarkable for overcoming physical and mental fatigue as well as brain fog. Although I am not keen on the inclusion of mannitol, I can overlook that.
7.5
Clarity in thinking
1 people found this helpful
After weeks of this B12 supplement, I've noticed greater clarity in my thinking and an uplift in my mood, especially beneficial for my chemotherapy-induced neuropathy.
9
Hair and nail improvement
10 people found this helpful
This product effectively raised my B12 levels from 270 to 960. I suffered from hair loss, but after a month of a high dosage B12 supplement, my levels improved significantly. I feel a surge of strength and clarity in my brain.
9
Calm through craziness
1 people found this helpful
This B12 gives me great energy and clears brain fog. I feel calmer during hectic days at work, making it the best B12 for me.
7.5
Repairing brain nerves
1 people found this helpful
I feel positive effects from this high-dose B12 supplement, particularly in helping repair brain nerves and improving my sleep quality. I'm keen to see more pronounced effects.
6
Boosts focus
Easy on the stomach with great quality. Methylcobalamin crosses the blood-brain barrier, which genuinely assists with focus and energy!
4
Vitamin B12's unclear impact on glioblastoma
We explored the effects of vitamin B12 (cobalamin) supplementation on a human glioblastoma cell line known as U-87 MG. Our main goal was to understand how cobalamin, particularly in combination with pyridoxine (vitamin B6), influences cell viability and the process of cell death, known as apoptosis.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
Our approach involved treating the glioblastoma cells with varying concentrations of vitamin B12 over a period of 24 to 72 hours. We assessed the health of the cells and their progression through the cell cycle using methods like spectrophotometry and flow cytometry. We also measured specific proteins associated with cell survival and death through western blot analysis.
We found that while vitamin B6 supplementation led to decreased cell viability and increased signs of apoptosis, the role of vitamin B12 was less clear. It appeared that cobalamin did not significantly enhance or inhibit cell death on its own. However, when combined with pyridoxine, it seemed to reduce the cytotoxic effects of vitamin B6.
In summary, while vitamin B12 in isolation did not show a direct benefit for the glioblastoma cells in our study, its potential to influence the effects of other vitamins highlights a complex interaction deserving of further exploration.
References
- MartĂnez-Mendiola CA, Estrada JA, Zapi-ColĂn LĂ, Contreras-ChĂĄvez GG, Contreras I. Effect of pyridoxine or cobalamin supplementation on apoptosis and cell cycle progression in a human glioblastoma cell line. Int J Neurosci. 2024;134:1320. doi:10.1080/00207454.2023.2263815
