Lacticaseibacillus rhamnosus shows promiseIn Vitro and In Vivo Evaluation of Lacticaseibacillus rhamnosus GG and Bifidobacterium lactis Bb12 Against Avian Pathogenic Escherichia coli and Identification of Novel Probiotic-Derived Bioactive Peptides.
High relevance for poultry health
We explored the potential of Lacticaseibacillus rhamnosus GG as a probiotic treatment against avian pathogenic E. coli (APEC) in chickens. Our study showed that this probiotic significantly reduced APEC colonization in the chickens' cecum and improved the balance of gut microbiota over a 24-hour period.
Through laboratory evaluations, we detected novel bioactive peptides in the probiotic's culture supernatants, which may contribute to its antibacterial properties. While the results are promising for poultry health, more research is needed to optimize its use in real farming conditions.
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FOS-inulin aids Salmonella defenseEffects of fructooligosaccharide-inulin on Salmonella-killing and inflammatory gene expression in chicken macrophages.
Study closely examines FOS-inulin effects
Our study aimed to explore how a prebiotic called fructooligosaccharide (FOS)-inulin affects chickens' ability to combat food poisoning caused by Salmonella Enteritidis (SE). We focused on chicken macrophages, which are important immune cells that help eliminate this pathogen. Using an in vitro model, we treated the HD11 macrophage cell line with FOS-inulin and assessed its ability to phagocytose and kill SE, as well as the production of inflammatory markers.
The results were quite revealing. After treatment with FOS-inulin, we found that the HD11 cells contained significantly fewer viable SE compared to those that were untreated, suggesting that this prebiotic supports macrophage function in eliminating the bacteria. However, it’s worth noting that FOS-inulin did not appear to influence the overall phagocytosis process or the production of nitric oxide, a key player in the immune response.
Additionally, we observed that levels of IL-1β, an important inflammatory cytokine, were significantly reduced in macrophages treated with FOS-inulin. This indicates that FOS-inulin can help modulate the innate immune response, making it potentially beneficial for enhancing the body's defense against foodborne pathogens while also reducing excessive inflammation.
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Inulin aids gut health during infectionProbiotic/prebiotic correction for adverse effects of iron fortification on intestinal resistance to Salmonella infection in weaning mice.
Study highlights inulin's benefits
We investigated how inulin, a popular prebiotic, affects gut health during instances of food poisoning, particularly following iron fortification. In our study, we observed that iron fortification can increase diarrhea risk in weaning mice and, unfortunately, make them more susceptible to infections like Salmonella.
Mice given a diet with 500 ppm of iron showed more severe symptoms when infected, including weight loss and reduced appetite. However, we found that supplementing their diet with inulin for just seven days before infection significantly improved their health outcomes. The presence of inulin helped reduce the abundance of the harmful pathogen, Salmonella, in their intestines and alleviated gut damage caused by iron.
Our results suggest that inulin acts as a protective barrier, improving gut health by fostering a balance in gut microbiota during challenging times. This research highlights the potential of using inulin to mitigate the harmful side effects of iron supplementation, offering valuable insights for dietary considerations, particularly among vulnerable populations like children.
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