We explored the effectiveness of nattokinase, a natural enzyme known for its role in breaking down blood clots, by studying its production and activity in a lab setting. Nattokinase was extracted from Bacillus subtilis, and we introduced this gene into E. coli bacteria to produce the enzyme at high levels.
Through our process, we purified the active recombinant nattokinase and tested its ability to dissolve blood clots. Our results were promising; the enzyme exhibited a fibrinolytic activity of 58FU/mg, which is notably five times more effective than the currently used drug, orokinase.
We optimized the conditions for producing nattokinase, including factors like temperature and nutrient concentrations, resulting in an impressive yield of 1805.50 mg/L. This improved production method paves the way for scaling up nattokinase's use in treating cardiovascular diseases related to blood clots. The findings emphasize nattokinase's potential as an effective thrombolytic agent.
Eliminated headache
Most Useful Review
I highly recommend it! The swelling has gone down, and my blood is flowing well. I began taking it during the pandemic and since then, I've not had a headache, which is remarkable for me.