Overview

SCIENTIFIC SCORE
Possibly Effective
Based on 35 Researches
7.5
USERS' SCORE
Good
Based on 1 Review
8.4
Supplement Facts
Serving Size: 2 Soft Gels
Amount Per Serving
%DV
Calories
15
 
Total Fat
1.5 g
2%
Saturated Fat
0 g
0%
Trans Fat
0 g
†
Vitamin D3 (cholecalciferol)
25 mg (1000 IU)
167%
Total Omega-3s♦
1120 mg
†
EPA (Eicosapentaenoic Acid)
586 mg
†
DHA (Docosahexaenoic Acid)
456 mg
†

Top Medical Research Studies

9
CYP11A1, Autism Symptoms, Vitamin D3
We explored how overexpression of the CYP11A1 enzyme in the placenta can impact offspring and potentially lead to autism-like behaviors, particularly focusing on anxiety. Using a specialized murine model, we looked closely at how this genetic change affected social interactions and anxiety levels in the mice.

Our findings highlighted a significant increase in anxiety-related behaviors, which we measured through various tests like the open field and elevated plus maze assessments. Interestingly, we observed that supplementing with vitamin D3 led to noticeable improvements in these anxiety behaviors, suggesting a protective role for vitamin D3 against the adverse effects caused by altered hormone levels from the Cyp11a1 overexpression.

Additionally, we found that vitamin D3 treatment not only helped in behavioral aspects but also corrected underlying neuroinflammatory responses—specifically the activation of microglia in the brain. This suggests that addressing vitamin D levels during pregnancy could be a potential strategy to mitigate these changes and improve outcomes for offspring at risk of autism spectrum disorders.
Read More
We explored the effect of vitamin D3 supplementation on anxiety levels among elderly individuals diagnosed with prediabetes. In our study, we randomly assigned participants to receive either a weekly dose of 25,000 IU of vitamin D3 or a placebo, alongside general lifestyle recommendations. Over the course of 12 months, we measured anxiety levels using standardized assessments.

At both the six-month and twelve-month marks, the participants receiving vitamin D3 reported significantly lower anxiety levels compared to those who did not receive the supplementation. Specifically, scores measuring anxiety decreased from 38.02 to 32.35 in the vitamin D group, while control participants’ scores remained much higher throughout the study.

These results indicate that vitamin D3 could be a beneficial and safe option for alleviating anxiety in an older population already facing the challenges of prediabetes. The participants who had vitamin D deficiency or insufficiency at the start of the study experienced similar improvements, emphasizing the treatment's potential across various baseline vitamin D levels.

Overall, our findings support the idea that vitamin D supplementation can effectively help mitigate anxiety symptoms in older adults, and we believe further research is warranted to better understand the mechanisms at play.
Read More
9
We explored how coal dust exposure leads to anxiety and depression-like behaviors in mice, using a pneumoconiosis model that was established after 28 days of exposure. This model allows us to closely observe the effects of environmental factors on mental health. Through treating the mice with Vitamin D3 for three months, we aimed to see if this treatment could alleviate anxiety symptoms.

Our findings were quite revealing. Vitamin D3 treatment showed a significant positive effect; it reduced signs of anxiety and depression. Specifically, we saw improvements in tests designed to measure these behaviors, along with a decrease in markers of astrocyte activation—cells in the brain that can become overactive in stressful situations. Vitamin D3 also promoted better neuron protection and enhanced the distribution of brain-derived neurotrophic factor (BDNF), a crucial element for brain health.

This study suggests that Vitamin D might serve as a useful approach for managing mood disorders resulting from environmental stressors such as coal dust. These findings also highlight the importance of further exploring Vitamin D3 as a potential treatment avenue for anxiety exacerbated by particulate matter exposure.
Read More

Most Useful Reviews

7.5
Less effective
6 people found this helpful
I’ve taken a DHA supplement and prenatal vitamin since pregnancy. I wanted to try a cheaper fish oil and stop the prenatal. Unfortunately, this did not manage my anxiety nearly as effectively as the Rainbow Light brand. I plan to return to their product.
Read More

Medical Researches

SCIENTIFIC SCORE
Possibly Effective
Based on 35 Researches
7.5
  • All Researches
9
CYP11A1, Autism Symptoms, Vitamin D3
We explored how overexpression of the CYP11A1 enzyme in the placenta can impact offspring and potentially lead to autism-like behaviors, particularly focusing on anxiety. Using a specialized murine model, we looked closely at how this genetic change affected social interactions and anxiety levels in the mice.

Our findings highlighted a significant increase in anxiety-related behaviors, which we measured through various tests like the open field and elevated plus maze assessments. Interestingly, we observed that supplementing with vitamin D3 led to noticeable improvements in these anxiety behaviors, suggesting a protective role for vitamin D3 against the adverse effects caused by altered hormone levels from the Cyp11a1 overexpression.

Additionally, we found that vitamin D3 treatment not only helped in behavioral aspects but also corrected underlying neuroinflammatory responses—specifically the activation of microglia in the brain. This suggests that addressing vitamin D levels during pregnancy could be a potential strategy to mitigate these changes and improve outcomes for offspring at risk of autism spectrum disorders.
Read More
We explored the effects of vitamin D3 on anxiety levels in rats subjected to chronic unpredictable mild stress. In our study, we used a total of 40 male Wistar rats, dividing them into five groups to analyze various treatments. These groups included a control group, a stress group, and groups receiving either vitamin D3, fluoxetine (an antidepressant), or a combination of both.

The chronic mild stress model we applied involved exposing the rats to different physical and social stressors over 21 days. Throughout the study, we monitored changes in behavior through established tests, such as the sucrose preference test and the forced swimming test, while also measuring stress hormone levels.

Our findings revealed that vitamin D3 had a protective effect against anxiety and depressive behaviors similar to fluoxetine, particularly in certain behavioral tests. In groups receiving vitamin D3, we observed lower levels of stress hormone corticosterone compared to those only exposed to stress.

Overall, we concluded that vitamin D3 can offer significant support against anxiety when faced with stress. While it was effective, we noted that the combination with fluoxetine complicates isolating the specific impact of vitamin D3 alone.
Read More
We explored the effect of vitamin D3 supplementation on anxiety levels among elderly individuals diagnosed with prediabetes. In our study, we randomly assigned participants to receive either a weekly dose of 25,000 IU of vitamin D3 or a placebo, alongside general lifestyle recommendations. Over the course of 12 months, we measured anxiety levels using standardized assessments.

At both the six-month and twelve-month marks, the participants receiving vitamin D3 reported significantly lower anxiety levels compared to those who did not receive the supplementation. Specifically, scores measuring anxiety decreased from 38.02 to 32.35 in the vitamin D group, while control participants’ scores remained much higher throughout the study.

These results indicate that vitamin D3 could be a beneficial and safe option for alleviating anxiety in an older population already facing the challenges of prediabetes. The participants who had vitamin D deficiency or insufficiency at the start of the study experienced similar improvements, emphasizing the treatment's potential across various baseline vitamin D levels.

Overall, our findings support the idea that vitamin D supplementation can effectively help mitigate anxiety symptoms in older adults, and we believe further research is warranted to better understand the mechanisms at play.
Read More
9
We explored how coal dust exposure leads to anxiety and depression-like behaviors in mice, using a pneumoconiosis model that was established after 28 days of exposure. This model allows us to closely observe the effects of environmental factors on mental health. Through treating the mice with Vitamin D3 for three months, we aimed to see if this treatment could alleviate anxiety symptoms.

Our findings were quite revealing. Vitamin D3 treatment showed a significant positive effect; it reduced signs of anxiety and depression. Specifically, we saw improvements in tests designed to measure these behaviors, along with a decrease in markers of astrocyte activation—cells in the brain that can become overactive in stressful situations. Vitamin D3 also promoted better neuron protection and enhanced the distribution of brain-derived neurotrophic factor (BDNF), a crucial element for brain health.

This study suggests that Vitamin D might serve as a useful approach for managing mood disorders resulting from environmental stressors such as coal dust. These findings also highlight the importance of further exploring Vitamin D3 as a potential treatment avenue for anxiety exacerbated by particulate matter exposure.
Read More
9
Vitamin D3 reduces anxiety in withdrawal
We examined the effects of vitamin D3 on anxiety in mice experiencing nicotine withdrawal. To conduct our research, we divided male C57/BL6 mice into four distinct groups: those receiving a vehicle, those receiving nicotine alone, those receiving vitamin D3 alone, and those receiving both nicotine and vitamin D3.

During the six-week study, the mice were given nicotine through their drinking water and vitamin D3 in their feed. When the withdrawal phase kicked in, we observed that mice treated with vitamin D3 demonstrated significantly reduced anxiety-like behaviors. This was measured using open-field tests and marble burying tests, where these mice spent more time in the central area and buried fewer marbles compared to those that did not receive vitamin D3.

Additionally, our study revealed that vitamin D3 supplementation reduced hippocampal NR2A expression, which may be linked to lowering anxiety during nicotine withdrawal. These findings suggest that vitamin D3 supplementation could be a promising dietary intervention for people dealing with anxiety related to quitting smoking.
Read More

User Reviews

USERS' SCORE
Good
Based on 1 Review
8.4
  • All Reviews
  • Positive Reviews
  • Negative Reviews
7.5
Less effective
6 people found this helpful
I’ve taken a DHA supplement and prenatal vitamin since pregnancy. I wanted to try a cheaper fish oil and stop the prenatal. Unfortunately, this did not manage my anxiety nearly as effectively as the Rainbow Light brand. I plan to return to their product.
Read More

Frequently Asked Questions

No FAQs are available for this product and symptom.

References

  1. Gizzi G, Fiorani F, Cataldi S, Mandarano M, Delvecchio E, et al. Exploring the Influence of / Polymorphic Variants on Adolescent Mental Health and Response to Vitamin D Supplementation in Embryonic Hippocampal Cell Lines. Genes (Basel). 2024;15. 10.3390/genes15070913
  2. Yin H, Zhang J, Chen Y, Guo J, Li Q, et al. Placenta-specific CYP11A1 overexpression lead to autism-like symptom in offspring with altered steroid hormone biosynthesis in the placenta-brain axis and rescued by vitamin D intervention. Brain Behav Immun. 2024;121:13. 10.1016/j.bbi.2024.07.012
  3. Renteria KM, Constantine E, Teoh CM, Cooper A, Lozano N, et al. Combination of vitamin D and fructooligosaccharides upregulates colonic vitamin D receptor in C57BL/6J mice and affects anxiety-related behavior in a sex-specific manner. Nutr Res. 2024;125:16. 10.1016/j.nutres.2024.02.003
  4. Al-Ramadhan FR, Abulmeaty MMA, Alquraishi M, Razak S, Alhussain MH. Effect of Vitamin D3 on Depressive Behaviors of Rats Exposed to Chronic Unpredictable Mild Stress. Biomedicines. 2023;11. 10.3390/biomedicines11082112
  5. Lapmanee S, Bhubhanil S, Sriwong S, Yuajit C, Wongchitrat P, et al. Oral calcium and vitamin D supplements differentially alter exploratory, anxiety-like behaviors and memory in male rats. PLoS One. 2023;18:e0290106. 10.1371/journal.pone.0290106
  6. Pereira ADS, Miron VV, Castro MFV, Bottari NB, Assmann CE, et al. Neuromodulatory effect of the combination of metformin and vitamin D triggered by purinergic signaling in type 1 diabetes induced-rats. Mol Cell Endocrinol. 2023;563:111852. 10.1016/j.mce.2023.111852
  7. Zaromytidou E, Koufakis T, Dimakopoulos G, Drivakou D, Konstantinidou S, et al. Vitamin D Alleviates Anxiety and Depression in Elderly People with Prediabetes: A Randomized Controlled Study. Metabolites. 2022;12. 10.3390/metabo12100884
  8. Tokarchuk A, Abenavoli L, Kobyliak N, Khomenko M, Revun M, et al. Nutrition program, physical activity and gut microbiota modulation: a randomized controlled trial to promote a healthy lifestyle in students with vitamin D3 deficiency. Minerva Med. 2022;113:683. 10.23736/S0026-4806.22.07992-7
  9. Zou Y, Mu M, Zhang S, Li C, Tian K, et al. Vitamin D3 suppresses astrocyte activation and ameliorates coal dust-induced mood disorders in mice. J Affect Disord. 2022;303:138. 10.1016/j.jad.2022.02.026
  10. Guzek D, Kołota A, Lachowicz K, Skolmowska D, Stachoń M, et al. Influence of Vitamin D Supplementation on Mental Health in Diabetic Patients: A Systematic Review. Nutrients. 2021;13. 10.3390/nu13113678
  11. Wu B, Tao X, Liu C, Li H, Jiang T, et al. Vitamin D3 reduces hippocampal NR2A and anxiety in nicotine withdrawal mice. Transl Neurosci. 2021;12:273. 10.1515/tnsci-2020-0166
  12. Tsoukalas D, Zlatian O, Mitroi M, Renieri E, Tsatsakis A, et al. A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals. J Clin Med. 2021;10. 10.3390/jcm10040624
  13. Lang F, Ma K, Leibrock CB. 1,25(OH)D in Brain Function and Neuropsychiatric Disease. Neurosignals. 2019;27:40. 10.33594/000000182
  14. Verde Z, Giaquinta A, Sainz CM, Ondina MD, Araque AF. Bone Mineral Metabolism Status, Quality of Life, and Muscle Strength in Older People. Nutrients. 2019;11. 10.3390/nu11112748
  15. Fedotova JO. Vitamin D treatment differentially affects anxiety-like behavior in the old ovariectomized female rats and old ovariectomized female rats treated with low dose of 17β-estradiol. BMC Med Genet. 2019;20:49. 10.1186/s12881-019-0774-2
  16. Fazelian S, Amani R, Paknahad Z, Kheiri S, Khajehali L. Effect of Vitamin D Supplement on Mood Status and Inflammation in Vitamin D Deficient Type 2 Diabetic Women with Anxiety: A Randomized Clinical Trial. Int J Prev Med. 2019;10:17. 10.4103/ijpvm.IJPVM_174_18
  17. Demin KA, Kolesnikova TO, Galstyan DS, Krotova NA, Ilyin NP, et al. The Utility of Prolonged Chronic Unpredictable Stress to Study the Effects of Chronic Fluoxetine, Eicosapentaenoic Acid, and Lipopolysaccharide on Anxiety-Like Behavior and Hippocampal Transcriptomic Responses in Male Rats. J Neurosci Res. 2025;103:e70025. 10.1002/jnr.70025
  18. Strekalova T, Radford-Smith D, Dunstan IK, Gorlova A, Svirin E, et al. Omega-3 alleviates behavioral and molecular changes in a mouse model of stress-induced juvenile depression. Neurobiol Stress. 2024;31:100646. 10.1016/j.ynstr.2024.100646
  19. Nguyen N, Woodside DB, Lam E, Quehenberger O, German JB, et al. Fatty Acids and Their Lipogenic Enzymes in Anorexia Nervosa Clinical Subtypes. Int J Mol Sci. 2024;25. 10.3390/ijms25105516
  20. Wang HF, Liu WC, Zailani H, Yang CC, Chen TB, et al. A 12-week randomized double-blind clinical trial of eicosapentaenoic acid intervention in episodic migraine. Brain Behav Immun. 2024;118:459. 10.1016/j.bbi.2024.03.019
  21. Xue Y, Wang L, Liu T, Zhao T, Xie K, et al. Omega-3 polyunsaturated fatty acids supplementation improves memory in first-diagnosed, drug-naĂŻve patients with depression: Secondary analysis of data from a randomized controlled trial. J Affect Disord. 2024;350:403. 10.1016/j.jad.2024.01.149
  22. Chang CH, Wu HC, Hsieh YR, Lai WD, Tung TH, et al. Modulatory effect of n-3 polyunsaturated fatty acids on depressive-like behaviors in rats with chronic sleep deprivation: potential involvement of melatonin receptor pathway and brain lipidome. Food Funct. 2023;14:5977. 10.1039/d3fo01452e
  23. Kelaiditis CF, Gibson EL, Dyall SC. Effects of long-chain omega-3 polyunsaturated fatty acids on reducing anxiety and/or depression in adults; A systematic review and meta-analysis of randomised controlled trials. Prostaglandins Leukot Essent Fatty Acids. 2023;192:102572. 10.1016/j.plefa.2023.102572
  24. Kishi T, Sakuma K, Iwata N. Mitochondrial modulators for obsessive-compulsive and related disorders: a systematic review and meta-analysis. Transl Psychiatry. 2022;12:263. 10.1038/s41398-022-02026-5
  25. Mengelberg A, Leathem J, Podd J, Hill S, Conlon C. The effects of docosahexaenoic acid supplementation on cognition and well-being in mild cognitive impairment: A 12-month randomised controlled trial. Int J Geriatr Psychiatry. 2022;37. 10.1002/gps.5707
  26. Neto J, Jantsch J, de Oliveira S, Braga MF, Castro LFDS, et al. DHA/EPA supplementation decreases anxiety-like behaviour, but it does not ameliorate metabolic profile in obese male rats. Br J Nutr. 2022;128:964. 10.1017/S0007114521003998
  27. Wang CC, Du L, Shi HH, Ding L, Yanagita T, et al. Dietary EPA-Enriched Phospholipids Alleviate Chronic Stress and LPS-Induced Depression- and Anxiety-Like Behavior by Regulating Immunity and Neuroinflammation. Mol Nutr Food Res. 2021;65:e2100009. 10.1002/mnfr.202100009
  28. Demers G, Roy J, Machuca-Parra AI, Dashtehei Pour Z, Bairamian D, et al. Fish oil supplementation alleviates metabolic and anxiodepressive effects of diet-induced obesity and associated changes in brain lipid composition in mice. Int J Obes (Lond). 2020;44:1936. 10.1038/s41366-020-0623-6
  29. Nakajima S, Fukasawa K, Gotoh M, Murakami-Murofushi K, Kunugi H. Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level. Int J Obes (Lond). 2020;44:727. 10.1038/s41366-019-0468-z
  30. Provensi G, Schmidt SD, Boehme M, Bastiaanssen TFS, Rani B, et al. Preventing adolescent stress-induced cognitive and microbiome changes by diet. Proc Natl Acad Sci U S A. 2019;116:9644. 10.1073/pnas.1820832116
  31. Li Y, Hua L, Ran Q, Gu J, Bao Y, et al. Plasma Polyunsaturated Fatty Acid Levels and Mental Health in Middle-Aged and Elderly Adults. Nutrients. 2024;16. 10.3390/nu16234065
  32. Gajdošová L, Katrenčíková B, Borbélyová V, Muchová J. The Effect of Omega-3 Fatty Acid Supplementation and Exercise on Locomotor Activity, Exploratory Activity, and Anxiety-Like Behavior in Adult and Aged Rats. Physiol Res. 2024;73:461.
  33. Zambrano E, Rodríguez-González GL, Reyes-Castro LA, Bautista CJ, Castro-Rodríguez DC, et al. DHA Supplementation of Obese Rats throughout Pregnancy and Lactation Modifies Milk Composition and Anxiety Behavior of Offspring. Nutrients. 2021;13. 10.3390/nu13124243
  34. Tang Q, Li S, Fang C, Yu H. Evaluating the reparative effects and the mechanism of action of docosahexaenoic acid on azithromycin-induced lipid metabolism dysfunction. Food Chem Toxicol. 2022;159:112699. 10.1016/j.fct.2021.112699
  35. Ponomarenko AI, Tyrtyshnaia AA, Pislyagin EA, Dyuizen IV, Sultanov RM, et al. N-docosahexaenoylethanolamine reduces neuroinflammation and cognitive impairment after mild traumatic brain injury in rats. Sci Rep. 2021;11:756. 10.1038/s41598-020-80818-9
image